How do you separate plant pigments by paper chromotography? - plant pigments chromotography lab report
Since the question is, how does it work? I suppose to send some background information for my lab, but we do not know where to start. Thanks in advance
Sunday, January 10, 2010
Plant Pigments Chromotography Lab Report How Do You Separate Plant Pigments By Paper Chromotography?
3 comments:
First, there are so many different pigments in plants, not only "green" is seen with the naked eye (although the green pigment chlorophyll in most plants is important). Other kerotins pigments (orange like carrots) and xanthophyll.
These are the basics. The second part is to understand the structure of the pigments. Each pigment has a different chemical composition to be separated and may, by taking note of these properties.
Well established for the first time a long strip of paper, draw a line 1 cm from the edge, and the cut edges in a triangle. Rub a sheet with a coin or something to the pigmented parts of the rental or. Remove transfer paper to create a line. When the tip is placed in a solvent through the paper up and move slowly absorbed. When this happens, the solvent is separated from the pigment, because of their chemical properties. For example, do the green pigment chlorophyll, do not go too far because it is large and consists of its commitments on paper to integrate. Other pigments were separated from the green and eventully should the bands of yellow and orange.
The key here is the chemistry behind paper chromatography, but if you are (ie in the class of lesser quality, 4-10 degrees), then I recommend you find a site that can explain better. If you are in the upper classes, I suggest you look for the chemical properties of pigments, is Wikipedia a good leader.
Grind the leaves in alcohol, filtering the mixture of waste to be free. One end of a strip of paper, water color, weather, and even a strip of coffee filter Mixure alcohol, and you keep the paper ... It will also flow various pigments for paper, but at the same pace ... to indicate different bands of color. This is called paper chromatography.
Ooh, we have a laboratory in this ... reads as follows:
Plant pigments are placed on chromatography paper ... is, .. in a solvent Travel the role of the solvent, which leads to the same pigments. Pigments are made at different rates because they are soluble and are attracted to the role of various degrees. The most common carotene in plants of beta-carotene. carried out near the surface of the solvent, because it is highly soluble, and no hydrogen bonds. Xanthophyll is another, but contains no oxygen. will continue to push, because it is less soluble, and is constrained by hydrogen bonds. Chlorophyll with oxygen and nitrogen, and more closely linked to paper.
Post a Comment